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anti human cd9 beads  (Exosome Diagnostics)

 
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    Structured Review

    Exosome Diagnostics anti human cd9 beads
    FACS-based characterization of isolated EVs (second round). A FACS histograms depicting the relative fluorescence/marker intensity of EV preparation 2.1 (black line) against unstained EV particle control (grey line). B Corresponding marker expression in HCT116 cells (extracellular staining for <t>CD9,</t> CD63, and CD81 and intracellular staining for Alix, TSG101, and calnexin). C, E Mean fluorescence intensity (MFI) raw values of CD63 (C) and CD81 (E) marker expression from laboratories 2.1–2.4. D, F MFI values per particle concentration of CD63 (D) and CD81 (F; left y axis) against the respective particle concentration per ml CCM (right y axis).
    Anti Human Cd9 Beads, supplied by Exosome Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+cd9+beads/Human+CD9+Antibody/pmc07923922-142-5-11
    Average 90 stars, based on 9 article reviews
    anti human cd9 beads - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation"

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation

    Journal: Transfusion Medicine and Hemotherapy

    doi: 10.1159/000508712

    FACS-based characterization of isolated EVs (second round). A FACS histograms depicting the relative fluorescence/marker intensity of EV preparation 2.1 (black line) against unstained EV particle control (grey line). B Corresponding marker expression in HCT116 cells (extracellular staining for CD9, CD63, and CD81 and intracellular staining for Alix, TSG101, and calnexin). C, E Mean fluorescence intensity (MFI) raw values of CD63 (C) and CD81 (E) marker expression from laboratories 2.1–2.4. D, F MFI values per particle concentration of CD63 (D) and CD81 (F; left y axis) against the respective particle concentration per ml CCM (right y axis).
    Figure Legend Snippet: FACS-based characterization of isolated EVs (second round). A FACS histograms depicting the relative fluorescence/marker intensity of EV preparation 2.1 (black line) against unstained EV particle control (grey line). B Corresponding marker expression in HCT116 cells (extracellular staining for CD9, CD63, and CD81 and intracellular staining for Alix, TSG101, and calnexin). C, E Mean fluorescence intensity (MFI) raw values of CD63 (C) and CD81 (E) marker expression from laboratories 2.1–2.4. D, F MFI values per particle concentration of CD63 (D) and CD81 (F; left y axis) against the respective particle concentration per ml CCM (right y axis).

    Techniques Used: Isolation, Fluorescence, Marker, Control, Expressing, Staining, Concentration Assay

    Related Articles

    Isolation:

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
    Article Snippet: Fluorescence-Activated Cell Sorting Analysis FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).Fluorescence-Activated Cell Sorting Analysis FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).. CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
    Article Snippet: FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).. CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).

    Fluorescence:

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
    Article Snippet: Fluorescence-Activated Cell Sorting Analysis FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).Fluorescence-Activated Cell Sorting Analysis FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).. CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
    Article Snippet: FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).. CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).

    Marker:

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
    Article Snippet: Fluorescence-Activated Cell Sorting Analysis FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).Fluorescence-Activated Cell Sorting Analysis FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).. CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
    Article Snippet: FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).. CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).

    Control:

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
    Article Snippet: Fluorescence-Activated Cell Sorting Analysis FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).Fluorescence-Activated Cell Sorting Analysis FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).. CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
    Article Snippet: FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).. CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).

    Expressing:

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
    Article Snippet: Fluorescence-Activated Cell Sorting Analysis FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).Fluorescence-Activated Cell Sorting Analysis FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).. CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
    Article Snippet: FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).. CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).

    Staining:

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
    Article Snippet: Fluorescence-Activated Cell Sorting Analysis FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).Fluorescence-Activated Cell Sorting Analysis FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).. CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
    Article Snippet: FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).. CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).

    Concentration Assay:

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
    Article Snippet: Fluorescence-Activated Cell Sorting Analysis FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).Fluorescence-Activated Cell Sorting Analysis FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).. CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation
    Article Snippet: FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).FACS measurement of HCT116-derived EVs and their parental cells was performed with BD FACS Canto II, using BD FACSDiva software (BD Biosciences).. HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).. CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).CD9 beads (20 µL) were washed with 1 mL of assay buffer (PBS + 0.1% BSA sterile-filtered 0.22 µm; BSA, Carl Roth).



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    FACS-based characterization of isolated EVs (second round). A FACS histograms depicting the relative fluorescence/marker intensity of EV preparation 2.1 (black line) against unstained EV particle control (grey line). B Corresponding marker expression in HCT116 cells (extracellular staining for <t>CD9,</t> CD63, and CD81 and intracellular staining for Alix, TSG101, and calnexin). C, E Mean fluorescence intensity (MFI) raw values of CD63 (C) and CD81 (E) marker expression from laboratories 2.1–2.4. D, F MFI values per particle concentration of CD63 (D) and CD81 (F; left y axis) against the respective particle concentration per ml CCM (right y axis).
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    FACS-based characterization of isolated EVs (second round). A FACS histograms depicting the relative fluorescence/marker intensity of EV preparation 2.1 (black line) against unstained EV particle control (grey line). B Corresponding marker expression in HCT116 cells (extracellular staining for <t>CD9,</t> CD63, and CD81 and intracellular staining for Alix, TSG101, and calnexin). C, E Mean fluorescence intensity (MFI) raw values of CD63 (C) and CD81 (E) marker expression from laboratories 2.1–2.4. D, F MFI values per particle concentration of CD63 (D) and CD81 (F; left y axis) against the respective particle concentration per ml CCM (right y axis).
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    Image Search Results


    FACS-based characterization of isolated EVs (second round). A FACS histograms depicting the relative fluorescence/marker intensity of EV preparation 2.1 (black line) against unstained EV particle control (grey line). B Corresponding marker expression in HCT116 cells (extracellular staining for CD9, CD63, and CD81 and intracellular staining for Alix, TSG101, and calnexin). C, E Mean fluorescence intensity (MFI) raw values of CD63 (C) and CD81 (E) marker expression from laboratories 2.1–2.4. D, F MFI values per particle concentration of CD63 (D) and CD81 (F; left y axis) against the respective particle concentration per ml CCM (right y axis).

    Journal: Transfusion Medicine and Hemotherapy

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation

    doi: 10.1159/000508712

    Figure Lengend Snippet: FACS-based characterization of isolated EVs (second round). A FACS histograms depicting the relative fluorescence/marker intensity of EV preparation 2.1 (black line) against unstained EV particle control (grey line). B Corresponding marker expression in HCT116 cells (extracellular staining for CD9, CD63, and CD81 and intracellular staining for Alix, TSG101, and calnexin). C, E Mean fluorescence intensity (MFI) raw values of CD63 (C) and CD81 (E) marker expression from laboratories 2.1–2.4. D, F MFI values per particle concentration of CD63 (D) and CD81 (F; left y axis) against the respective particle concentration per ml CCM (right y axis).

    Article Snippet: HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).

    Techniques: Isolation, Fluorescence, Marker, Control, Expressing, Staining, Concentration Assay

    FACS-based characterization of isolated EVs (second round). A FACS histograms depicting the relative fluorescence/marker intensity of EV preparation 2.1 (black line) against unstained EV particle control (grey line). B Corresponding marker expression in HCT116 cells (extracellular staining for CD9, CD63, and CD81 and intracellular staining for Alix, TSG101, and calnexin). C, E Mean fluorescence intensity (MFI) raw values of CD63 (C) and CD81 (E) marker expression from laboratories 2.1–2.4. D, F MFI values per particle concentration of CD63 (D) and CD81 (F; left y axis) against the respective particle concentration per ml CCM (right y axis).

    Journal: Transfusion Medicine and Hemotherapy

    Article Title: Inter-Laboratory Comparison of Extracellular Vesicle Isolation Based on Ultracentrifugation

    doi: 10.1159/000508712

    Figure Lengend Snippet: FACS-based characterization of isolated EVs (second round). A FACS histograms depicting the relative fluorescence/marker intensity of EV preparation 2.1 (black line) against unstained EV particle control (grey line). B Corresponding marker expression in HCT116 cells (extracellular staining for CD9, CD63, and CD81 and intracellular staining for Alix, TSG101, and calnexin). C, E Mean fluorescence intensity (MFI) raw values of CD63 (C) and CD81 (E) marker expression from laboratories 2.1–2.4. D, F MFI values per particle concentration of CD63 (D) and CD81 (F; left y axis) against the respective particle concentration per ml CCM (right y axis).

    Article Snippet: HCT116-derived EVs were captured on anti-human CD9 beads for flow detection (Exosome-Human CD9 beads; Thermo Fisher Scientific).

    Techniques: Isolation, Fluorescence, Marker, Control, Expressing, Staining, Concentration Assay